Test: GRE Subject Test: Biology

A student researcher wants to clone the human hemoglobin gene (HBB) into an expression vector so that he can express HBB in mouse cells and observe the resulting phenotype. 

1.

Which sequence of techniques will allow the student researcher to successfully clone the HBB gene?

None of the other answers

1. Ligate HBB

2. Use a restriction enzyme to digest the expression vector 

3. Amplify HBB and the digested expression vector via PCR

1. Use a restriction enzyme to digest HBB

2. Amplify the expression vector via PCR 

3. Ligate the digested HBB and vector

1. Use a restriction enzyme to digest the expression vector

2. Ligate HBB and the digested vector

3. Amplify HBB via PCR

1. Amplify HBB via PCR

2. Use a restriction enzyme to digest the expression vector

3. Ligate HBB and the digested vector

1/3 questions

0%

Access results and powerful study features!

Take 15 seconds to create an account.
Start now! Create your free account and get access to features like:
  • Full length diagnostic tests
  • Invite your friends
  • Access hundreds of practice tests
  • Monitor your progress over time
  • Manage your tests and results
  • Monitor the progress of your class & students
By clicking Create Account you agree that you are at least 13 years old and you agree to the Varsity Tutors LLC Terms of Use and Privacy Policy.
Learning Tools by Varsity Tutors