GRE Subject Test: Biochemistry, Cell, and Molecular Biology : Help with Biochemical Tagging

Study concepts, example questions & explanations for GRE Subject Test: Biochemistry, Cell, and Molecular Biology

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All GRE Subject Test: Biochemistry, Cell, and Molecular Biology Resources

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Example Questions

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Example Question #12 : Laboratory Practices

What methods were used in early DNA cloning to generate labeled DNA?

Possible Answers:

All of these

Nick translation

PCR generation of a new strand with a radioactive label

Synthesis of complementary strands

End labeling

Correct answer:

All of these

Explanation:

These are all possible methods for generating a labeled DNA probe that is easier to visualize in a heterogeneous sample. In nick translation, DNase is used to nick the DNA of interests, then DNA Polymerase I replaces some nucleotides with their labeled analogues. End labeling involves the use of labeled nucleotides that prevent further elongation at the ends of the DNA molecule. PCR with a radioactive label is another method of cloning labeled DNA.

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All GRE Subject Test: Biochemistry, Cell, and Molecular Biology Resources

1 Diagnostic Test 201 Practice Tests Question of the Day Flashcards Learn by Concept
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