Recent Tutoring Session Reviews
"We covered multiplying and dividing fractions and finding the reciprocals of a fraction; then he completed his atmospheric crossword puzzle. I gave him some instructions for organizing his book. We also reviewed for his math test on Wednesday. He grasped the concepts; however, he made simple errors from doing the math in his head."
"The student and I did a couple of worksheets to prepare him for a test on ionic compounds the next day. He rapidly picked up the basic concepts, and we spent most of the time reviewing to make sure he was perfectly prepared for the quiz."
"In this session, the student and I worked on an activity emulating the mechanics of recombinant DNA in plasmids for use in biotechnology. He had gotten started in class by cutting out and taping together strips of paper marked with two preset sequences of DNA. One set came in five strips to be mixed-and-matched which would form his plasmid with three sites of antibiotic resistance and an origin of replication. The other was a source of foreign DNA with a specific gene to be replicated. To these strands, we used cutouts representing restriction enzymes to identify sites where the two sources of DNA would be cut to form sticky ends. I challenged him to describe to me why it was important to have one cut in the plasmid but two in the strand of DNA. We then made the appropriate cuts, discussed the importance of ligase, and taped together our recombinant DNA with the foreign DNA inserted. We finished the session by talking through some questions about how we could use antibiotics to test whether bacteria had taken up the plasmid and what to do with this plasmid for industrial biotechnological purposes."