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Example Question #1 : Mixed Inhibition
A mixed inhibitor was found to bind unequally to an enzyme and its enzyme-substrate complex. It has a of for the enzyme, and a of for the enzyme-substrate complex.
If the inhibitor decreased by a factor of 3, what concentration of enzyme was used?
The answer cannot be determined without knowing the change in the enzyme's
Recall that the change in is only effected by the binding affinity to the enzyme substrate complex. We can represent the effect on the by this inhibitor by the following equation.
Since the apparent decreased by a factor of 3, we know that is 3.
Solve for in the following equation:
Above, is of the enzyme substrate complex, which is .